Tuning RNA Flexibility with Helix Length and Junction Sequence
نویسندگان
چکیده
منابع مشابه
A deoxyribozyme that forms a three-helix-junction complex with its RNA substrates and has general RNA branch-forming activity.
We recently used in vitro selection to identify 7S11, a deoxyribozyme that synthesizes 2',5'-branched RNA. The 7S11 DNA enzyme mediates the nucleophilic attack of an adenosine 2'-hydroxyl group at a 5'-triphosphate, forming 2',5'-branched RNA in a reaction that resembles the first step of in vivo RNA splicing. Here, we describe 7S11 characterization experiments that have two important implicati...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2015
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2015.10.039